Table 2. Enzyme kinetic parameters of recombinant CYP3A29 and its mutants oxidizing nifedipine.

Enzymes	SRS	V max (nmol/min/nmol P450)	K m (µmol/L)	CL int (mL/nmol P450/min)	n
Wild-type		8.53±0.69	15.68±2.96	0.54±0.03	1.10
R105A	SRS1	8.42±0.71	14.60±3.07	0.58±0.07	1.09
R106A	SRS1	9.86±0.37*	10.63±0.93*	0.93±0.02**	1.34
F108A	SRS1	7.54±0.57	13.43±2.37	0.56±0.03	1.26
S119A	SRS1	11.03±0.85*	24.15±3.68*	0.45±0.01**	1.41
K212A		10.02±0.89	21.71±1.83*	0.46±0.03*	1.48
F213A		8.45±0.61	14.50±2.45	0.58±0.04	1.25
F215A		9.46±1.03	17.67±4.45	0.53±0.08	1.18
R372A	SRS5	8.58±0.78	15.88±3.60	0.54±0.05	1.07
E374A	SRS5	8.48±0.72	17.05±3.08	0.50±0.05	1.41

Note: The values are expressed as means ± standard deviations of the results of three independent experiments. n indicates the Hill coefficients.

**(p<0.01) and *(p<0.05) indicate statistically significant difference between the wild-type and the mutant.