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. 2014 Sep 3;9(9):e106859. doi: 10.1371/journal.pone.0106859

Figure 5. Regionally extensive micro-vesiculation within epithelial crypts on the tonsil of the soft palate at 78 hpi detected by multichannel immunofluorescence.

Figure 5

A) Intra-epithelial microvesicles containing large quantities of FMDV structural (VP1; red) and non-structural (3D; turquoise) protein co-localized with cytokeratin (green) positive epithelial cells. 10× magnification, scale bar 100 µm. B) Serial section of region identified in A. FMDV VP1 protein (red) detected within cytokeratin (green) positive epithelial cells encircling an intra-epithelial microvesicle with acantholytic FMDV VP1/cytokeratin double positive cells detected within the vesicle lumina. CD172a (turquoise) and CD8 (purple; presumptive NK cells) leukocytes detected within and around the epithelial lesion, but without co-localization. 20× magnification, scale bar 50 µm. C) Cytokeratin-18 (turquoise) expressing M-cells in close proximity of FMDV VP1 (red) infected cytokeratin (green) positive epithelial cells within the tonsil of the soft palate. 40× magnification, scale bar 100 µm.