Table 2. Tissue distribution of FMDV during viremic phase of infection following IOP inoculation.
| 24 HPI | 48 HPI | 78 HPI | Mean GCN | ||||
| Animal ID | 34257 | 34256 | 34258 | 34255 | 37404 | 37405 | |
| FMDV RNA copies/µl in serum (log10) | 1.02 | 2.69 | 6.53 | 7.17 | 6.14 | 5.83 | |
| Oral cavity/oropharynx | |||||||
| Tongue | 2.81 | 4.63 | 5.71 | 6.46 | 6.85 | 7.08 | 5.59 |
| Lingual tonsil | NA | NA | NA | NA | 5.78 | 5.45 | 5.61 |
| Paraepiglottic tonsil | 5.71 | 6.29 | 5.59 | 5.30 | 5.91 | 5.03 | 5.64 |
| Tonsil of the soft palate | + | + | 4.78 | 4.22 | 6.05 | 5.95 | 5.25 |
| Nasal cavity/Nasopharynx | |||||||
| Dorsal soft palate -Rostral | 3.46 | 4.87 | 5.70 | 6.09 | 5.44 | 5.03 | 5.10 |
| Dorsal soft palate -Caudal | 5.10 | 4.02 | 6.32 | 6.13 | 5.53 | 4.72 | 5.30 |
| Nasal turbinates | - | 2.80 | 4.15 | 6.28 | 5.63 | 5.09 | 4.79 |
| Nasopharyngeal tonsil | 2.71 | + | 6.21 | 6.08 | 3.89 | 4.94 | 4.77 |
| Dorsal nasopharynx -Caudal | 3.96 | 4.98 | 5.62 | 5.78 | 5.58 | 5.28 | 5.20 |
| Dorsal nasopharynx -Rostral | 4.41 | 3.35 | 6.35 | 5.67 | 6.02 | 4.92 | 5.12 |
| Lungs/Trachea | |||||||
| Trachea | + | - | 5.69 | 6.98 | 4.66 | 5.71 | 5.76 |
| Proximal cranial lobe | - | 3.22 | 5.49 | 6.33 | 4.41 | 4.83 | 4.86 |
| Mid cranial lobe | - | 3.04 | 2.61 | 3.98 | 5.68 | 4.37 | 3.94 |
| Distal cranial lobe | - | 2.94 | 3.30 | 4.42 | 4.38 | 4.13 | 3.83 |
| Proximal mid lobe | - | 2.85 | 4.67 | 5.78 | 4.85 | 4.01 | 4.43 |
| Mid mid lobe | - | 3.33 | 3.74 | 5.34 | 4.80 | 4.37 | 4.32 |
| Distal mid lobe | - | 3.35 | 3.92 | 4.96 | 5.19 | 4.42 | 4.37 |
| Proximal caudal lobe | - | 2.67 | 3.87 | 6.96 | 3.83 | 4.72 | 4.41 |
| Mid caudal lobe | - | 3.02 | 3.87 | 5.81 | 4.22 | 4.50 | 4.28 |
| Distal caudal lobe | - | 3.34 | 3.93 | 5.33 | 4.81 | 4.80 | 4.44 |
| Visceral organs | |||||||
| Liver | - | + | + | 4.10 | 3.45 | 4.66 | 4.07 |
| Spleen | - | + | 2.99 | 4.79 | 5.93 | 4.97 | 4.67 |
| Kidney | - | + | 3.55 | 4.06 | NA | NA | 3.81 |
| Pancreas | - | + | 3.81 | 4.52 | - | 4.16 | |
| Peyer's Patches | + | + | 4.28 | 5.21 | 4.27 | 3.39 | 4.29 |
| Additional tissues | |||||||
| Medial Retropharyngeal LN | + | + | 3.28 | 4.49 | 5.61 | 3.40 | 4.19 |
| Submandibular LN | + | - | 3.87 | 4.19 | 5.03 | 2.81 | 3.98 |
| Hilar LN | - | + | 3.58 | 4.84 | 5.59 | 4.71 | 4.68 |
| Renal LN | - | - | 2.91 | 6.36 | 6.08 | 3.67 | 4.76 |
| Popliteal LN | + | 3.81 | 5.69 | 7.78 | 6.20 | 5.18 | 5.73 |
| Heart -right ventricle | - | - | 5.10 | 5.99 | 4.68 | 4.23 | 5.00 |
| Heart- left ventricle | - | - | 4.85 | 5.46 | 6.01 | 4.16 | 5.12 |
| Psoas Muscle | - | - | 4.44 | 5.79 | 3.70 | 6.04 | 4.99 |
| Semimembraneous muscle | + | - | 6.04 | 6.92 | 6.87 | 5.51 | 6.33 |
| Shoulder Muscle | - | + | 5.75 | 6.32 | 5.48 | 5.23 | 5.70 |
| Neck skin | - | - | 4.99 | 5.61 | 6.21 | 5.38 | 5.55 |
| Snout skin | - | - | 5.94 | 7.00 | 8.40 | 8.07 | 7.35 |
| Coronary band | - | - | 9.66 | 9.91 | 7.41 | 8.46 | 8.86 |
Numbers represent Log10 genome copy numbers (GCN)/mg of tissue. FMDV RNA content in serum is expressed as Log10 FMDV RNA copies/µl to facilitate comparison with viral content in tissues. Bold numbers indicate that samples were positive for both FMDV RNA (qRT-PCR) and virus isolation, (+) indicates that virus isolation was positive but FMDV RNA content was below the limit of detection, (-) indicates double negative samples. Limits of detection: tissue samples = 2.52 Log10 GCN/mg, serum≤0.1 Log10 GCN/µl (corresponding to an assay detection limit of 2.68 Log10 GCN/ml of serum).