Fig. 6.

hu14.18-IL2 is polarized between NKL and M21 cells and polarization is blocked with anti-CD25 blocking antibody. a NKL, b M21 cells, or c co-cultures of NKL and M21 cells were incubated with hu14.18-IL2FITC (as well as other treatments not pictured) for 25 min and then fixed onto a glass coverslip and visualized using confocal microscopy. Images were taken at 40×. Representative images of five separate experiments are shown in a–c. Data in c is a merged image showing 14.18-IL2-FITC (green) and actin (red) staining. Polarization of 14.18-IL2-FITC and actin is observed (yellow stain and yellow arrow) at the AIS between NKL and M21 cells. d Percent polarization was calculated by dividing the number of conjugates that displayed either hu14.18-IL2-FITC or hu14.18-FITC polarized at the synapse between two conjugates divided by the total number of conjugates. IC on the graph denotes hu14.18-IL2- FITC, while Ab denotes hu14.18-FITC. *P < 0.03