Figure 4. Enzymatic activity of the 17 strains. APIZYM and Rapid API 32A tests (Sysmex BioMérieux) were used for determination of enzymatic activities of each of the 17 strains. Cells from cultures grown on Eggerth-Gagnon blood agar plates for 48 h at 37 °C in an anaerobic chamber were suspended in saline and the turbidity was adjusted to 5–6 in the McFarland scale (approx. 1.5–1.9 × 10⁹ CFU/ml). Aliquots of 65 μl of the suspensions were inoculated into cupules in the strip. In the case of Rapid API 32A, the turbidity was matched to 4 in the McFarland scale and aliquots of 55 μl were applied to the cupules. The strips were incubated at 37 °C for 4 h and the reactions were determined according to the manufacturer’s instructions.