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Characterization of LCs generated from peripheral blood monocytes. iLCs were generated from isolated peripheral blood monocytes by incubating blood monocytes with GMCSF, TGF-β1, and IL-4. After 6 d, they were analyzed by flow cytometry. The cells were E-cadherin+ and approximately 50% were langerin+ (A), but immature (CD83−) (B). Day 11 iLCs (C) could be induced to mature, as measured by surface expression of CD83 and HLADR, when stimulated with either IL-36γ (D) or LPS (E) for 48 h.
