Skip to main content
. Author manuscript; available in PMC: 2014 Sep 4.
Published in final edited form as: Int J Cancer. 2010 Jul 15;127(2):313–320. doi: 10.1002/ijc.25049

Figure 5.

Figure 5

Knockdown microRNA-34a reduces PRIMA-1-induced apoptosis. H211 and H1155 cells were transfected with miRIDIAN hairpin inhibitor to inhibit expression of microRNA-34a or negative control. The transfected cells were treated with PRIMA-1 (25 μM for H211 and 100 μM for H1155) 24 hr posttransfection. After an additional incubation of 24 hr, the transfected and PRIMA-1-treated cells were harvested. TaqMan microRNA real-time PCR was used to confirm the specific knockdown of microRNA-34a in the H211 and H1155cells (a). The transfected and PRIMA-1-treated cells were then stained with Annexin V-FITC apoptosis kit and analyzed by fluorescence activated cell sorting (b). The apoptosis was also evaluated by Western blot analysis of PARP cleavage (c). Each value presented in this figure was an average value obtained from 3 independent analyses. The error bars show standard deviation. * indicates p < 0.05, 2-tailed t-test.