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. 2014 Aug 26;45(1):88. doi: 10.1186/s13567-014-0088-5

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© González-Warleta et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Histological findings in foetus/lamb brains positive to Neopora caninum PCR. A) Focus of gliosis with central area of coagulative to caseous necrosis in the grey matter (HE; Bar = 100 μm); B) Mild perivascular infiltration of mononuclear inflammatory cells in the white matter (HE; Bar = 200 μm); C) Intracellular cyst containing high number of bradyzoites. Note the absence of inflammation (HE; Bar = 25 μm); D) Immunohistochemical labelling of several protozoan tissue cysts in the grey matter. Note the absence of evident inflammatory infiltration in relation to the cysts (IHC; Bar = 300 μm) Inset: Detail of an immunolabelled tissue cyst (IHC; Bar = 15 μm).