Table 2.

Summary of DNA biophysical characterization of representative 2G QFP and corresponding control sequences

			Min. distance		Melting T 1/2°C*
Oligo	Sequence	Midpoint	5′	3′
A	GGA TGG GGT GGG GAG G	14813	27	0	80.0±1.5
Ac	AGA TGG AGT GGA GAG G				11.6±1.0
B	GGG GGA TGC GGG GG	13760	1	0	85.2±0.9
Bc	AGG AGA TGC AGG AG				14.0±2.0
C	GGA GGG TGG ATG G	5206	11	0	53.6±1.0
Cc	AGA GGG TAG ATG G				26.9±0.5

Oligonucleotides with QFP (A-C) or control sequences as indicted with (c) (mutations to ablate QFP are shown in bold) were tested for folding stability as reflected through their melting temperature, T_1/2. Midpoints of these sequences, their minimum distance to a 5′ and 3′ breakpoint, and T_1/2 are indicated. Melting temperatures were determined from CD melting curves as described in Methods section.