Table 2.
SNP call accuracy and SNP call rate of NF54 purified DNA
| ID | Parasite DNA (ng) | Call accuracy | Call rate |
|---|---|---|---|
| NF54-1 | 219 | 99.4% | 86.6% |
| NF54-2 | 212 | 99.5% | 81.5% |
| NF54-3 | 210 | 99.4% | 78.6% |
| NF54-4 | 203 | 99.4% | 73.8% |
| NF54-5 | 170 | 99.4% | 85.7% |
| NF54-6 | 168 | 99.4% | 77.3% |
| NF54-7 | 156 | 99.2% | 76.9% |
| NF54-8 | 152 | 99.3% | 69.0% |
| NF54 (WGA) | 222 | 99.6% | 91.6% |
| NF54 (WGA) | 138 | 99.5% | 85.1% |
NF54 reference strain DNA was typed at varying concentrations and call accuracy and call rate was calculated. Two samples underwent whole genome amplification (WGA) prior to genotyping and the concentration reflects post-WGA quantities.