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. 2014 Jun 25;307(5):C442–C454. doi: 10.1152/ajpcell.00379.2013

Fig. 1.

Fig. 1.

A: number of unique phosphopeptides identified by InsPecT and/or SEQUEST algorithms. B: effect of elevating NaCl for 1 h on abundance of proteins, as measured by iTRAQ. Abundance of only very few proteins changed significantly. C: phosphorylated (Y182) p38/MAPK14 peptide R.HTDDEMTGY*VATR.W(+2). Stable isotopic labeling of amino acids in cell culture (SILAC)-labeled isoforms were identified at 20.78 min. D: high NaCl increased abundance of R.HTDDEMTGY*VATR.W(+2). MS1 peaks are compared. E: phosphopeptides whose abundance was changed by high NaCl. High NaCl significantly increased abundance of 120 and decreased abundance of 207 of the 7,362 unique phosphopeptides that were quantified.