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. 2014 Jun 25;307(5):C442–C454. doi: 10.1152/ajpcell.00379.2013

Fig. 5.

Fig. 5.

p38/MAPK14 activity contributes to high-NaCl-induced increase in phosphorylation of STAT1 and HSP27/HSPB1. HEK 293 cells were preincubated for 30 min with the p38/MAPK14 inhibitor SB-203580 (8 μM) at 300 mosmol/kg; then the medium was changed for 1 h to an identical medium (control) or a medium still containing the inhibitor and with osmolality increased to 500 mosmol/kg by addition of NaCl. Immunoblots were prepared using anti-STAT1 and anti-phosphorylated (S727) STAT1 (A) or anti-HSP27/HSPB1 and anti-phosphorylated (S82) HSP27/HSPB1 antibodies (B). Top: representative Western blots. Bottom: means ± SE; n = 3. *P < 0.05 vs. control.