Fig. 4.

Evaluation of inflammation in the urinary bladder and central nervous system (CNS) after inoculation with MHV. A: hematoxylin and eosin staining of urinary bladder sections from a control mouse (top) and CIE mouse (bottom) showed no significant changes in the wall architecture. Scale bar = 200 μm. B: cytokine levels in the brain of control and CIE mice after 1 and 4 wk postinoculation as evaluated by multiplex ELISA. GM-CSF, granulocyte-macrophage colony-stimulating factor; IFN, interferon. C: concentration of cytokines in the spinal cord of control and infected mice. D: multiplex ELISA revealed no changes in the cytokine profile in the urinary bladder. *P ≤ 0.05 vs. the control group.