Fig. 2.

Genetic deletion of PKCε does not affect expression of the other 3 PKC isoforms presenting in pancreatic acinar cells. A: genotyping of PKCε mouse colonies with PCR. B: Western blot (W. Blot) analysis of PKCε and PKCδ in lysates of pancreatic acini isolated from WT mice and PKCε−/− mice. Samples were run in a single gel but were not continuous, as indicated by a line between lanes. C: Western blot analysis of PKC isoforms, ε, α, δ, and ζ in the lysates of pancreatic tissue collected from WT mice and PKCε−/− mice IP injected with saline (S) or cerulein (CR). GAPDH: for loading controls.