Fig. 4.

Deletion of PKCε attenuates necrosis and severity in cerulein pancreatitis. A: hematoxylin and eosin (H&E) staining of pancreatic tissue sections from mice with 7 hourly cerulein (or saline) injections. Each image was representative of at least 3 mice with similar results at each condition. Bars represent 20 μm. B: necrosis was measured on H&E-stained pancreatic tissue sections, as described in materials and methods. C and D: number of vacuole and inflammatory cells were counted on H&E-stained pancreatic tissue sections and expressed as percentage of total acinar cells. E: neutrophil infiltration within pancreas was assessed by measuring myeloperoxidase (MPO) activity in tissue homogenates and expressed as milliunits of MPO activity per milliliter homogenate (with same protein concentration). F: pancreatic edema (water content) was evaluated by measuring the wet-to-dry weight ratio of the pancreas tissue samples. Results in graphs (B–F) represent means ± SE (n = 3–6 mice) for each condition. **P < 0.01 or *P < 0.05 vs. WT with cerulein injections as indicated.