Fig. 9.

PKCε translocates to mitochondria in cerulein-induced pancreatitis. A: subcellular distribution of PKCε in early stage (30 min) of pancreatitis was determined in cytosolic and mitochondrial-enriched membrane fractions by Western blot analysis. The densities of PKCε bands were quantified and normalized to the saline controls. The blots were reprobed with an antibody against COX IV or LDH for loading controls and to assess the quality of separating membrane fractions from cytosolic fractions. Shown are representative Western blots from 3 independent experiments. B: colocalization of PKCε with VDAC1, a mitochondria marker in CR pancreatitis. Pancreatic tissue sections were double immunostained for PKCε (green) and VDAC1 (red), and secondary antibodies were conjugated with Alexa 488 or 594. Nuclei were counterstained with DAPI. Images were visualized under confocal microscope. The original magnification is ×63. Bar represents 10 μm. DIC, differential interference contrast.