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. 2014 Jun 26;6(8):1090–1104. doi: 10.15252/emmm.201404144

Figure 6. Pancreatic macrophages stimulate CXCR2 ligand production by the β-cells.

Figure 6

A–C   Macrophages were depleted by injection of clodronate liposomes or PBS liposomes (control) in female NOD mice between 1 and 3 weeks of age. Pancreatic islets were analyzed for the presence of neutrophils by flow cytometry (A), the production of CXCR2 ligands after culture by ELISA (B), or the expression of CXCL2 by the β-cells by flow cytometry (C). Data are representative (flow cytometry plot) or are median ± interquartile range (histogram or scatter plot) from three independent experiments each performed with two independent pools of three mice per group.

D   Pancreatic IL-1β-producing macrophages stimulate CXCL1/2 production by β-cells ex vivo. Min6 β-cells and sorted pancreatic or peritoneal macrophages from 3-week-old female NOD mice were cocultured for 48 h in a Transwell system. CXCL1 and CXCL2 productions in the culture supernatant were measured by ELISA. Data are median ± interquartile range from four independent experiments.