Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Apr 18;122(9):955–962. doi: 10.1289/ehp.1306911

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Publication of EHP lies in the public domain and is therefore without copyright. All text from EHP may be reprinted freely. Use of materials published in EHP should be acknowledged (for example, “Reproduced with permission from Environmental Health Perspectives”); pertinent reference information should be provided for the article from which the material was reproduced. Articles from EHP, especially the News section, may contain photographs or illustrations copyrighted by other commercial organizations or individuals that may not be used without obtaining prior approval from the holder of the copyright.

PMC Copyright notice

Effects of proteasome inhibition on acrolein-induced WRN protein degradation. WRN protein (A) and WRN and GAPDH mRNA (B) in HFL‑1 cells cultured in the absence (–) or presence (+) of 25 μM acrolein for 1, 2, or 3 days. (A) Protein levels were evaluated by immunoblot analysis (top); values are expressed as the relative densitometry ratio (WRN protein/β-actin), with each day 0 control regarded as 1.0 (bottom). (B) Steady-state levels of WRN and GAPDH mRNA were quantified by RT-PCR; values are expressed as the ratio of WRN to the GAPDH control. (C) WRN protein in HFL‑1 cells cultured without or with 25 μM acrolein for 48 hr and then incubated with 10 μM MG-132 or DMSO vehicle for 2 hr. (Top) Protein levels were evaluated by immunoblot analysis; (bottom) values are expressed as the relative densitometry ratio (WRN protein/β-actin), with the vehicle control regarded as 1.0. (D) WRN protein in transduced HFL‑1 cells [p53KD (p53-deficient) and pBH (control)] [p53KD (p53-deficient) or pBH (control)] cultured in the absence or presence of 25 μM acrolein for 2 days. (Top) Protein levels were evaluated by immunoblot analysis; (bottom) values are expressed as the relative densitometry ratio (WRN protein/ β-actin), with the pBH control regarded as 1.0. For A–D, data are expressed as mean ± SEM of three independent experiments. NS, not significant. *p < 0.05.