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. 2014 Apr 18;122(9):955–962. doi: 10.1289/ehp.1306911

Figure 4.

Figure 4

Effects of acrolein on population doubling (PD) and telomere length (TL). PD (A) and TL (B) in HFL‑1 cells cultured in the absence (–) or presence (+) of 10 μM acrolein. Relative TL is expressed as the mean ± SEM of triplicate samples at the PD upon acrolein-induced senescence (30 PD), the equivalent PD (30 PD) in control cells, and replicative senescence (46 PD) in control cells. (C) Immunoblot analysis of p53, p21, and WRN protein in HFL‑1 cells at day 10 and day 40 of culture in the absence (–) or presence (+) of 10 μM acrolein. PD (D) and and TL (E) in p53KD (p53-deficient) or pBH (control) cells cultured in the absence (–) or presence (+) of 10 μM acrolein. The relative TL is expressed as mean ± SEM of triplicate samples at the PD upon acrolein-induced cellular senescence in pBH cells (10 PD), and the equivalent PD in unexposed pBH cells and p53KD cells in the presence or absence of acrolein. (F) TL in p53KD cells cultured in the absence or presence of 10 μM acrolein. The relative TL is expressed as the mean ± SEM T/S ratio of triplicate samples at the PD upon replicative senescence (41 PD) and acrolein-induced senescence (31 PD) in p53KD cells, and the equivalent PD in unexposed p53KD cells. NS, not significant. *p < 0.05. **p < 0.01.