Figure 1.

DHT mediates paracrine regulation of EC function. A, Relative number of migrated HUVECs per Transwell, toward conditioned media (CM) from untreated control vs 48 hours DHT (40nM) treatment of fibroblasts from young and old men. Statistical analysis was performed using 2-way ANOVA with Bonferroni correction. *, P < .05 vs young control; n = 3 donors for young and old performed in duplicate over 3 individual experiments with data presented as means ± SEM. B, Relative number of tubule branch points per 4000 HUVECs treated for 24 hours with CM from control and 40nM DHT-treated young and old male fibroblasts. Statistical analysis was performed using 2-way ANOVA with Bonferroni correction. *, P < .05 vs young control; n = 3 donors for young and old performed in quadruplicate over 3 individual experiments with data presented as means ± SEM. Representative micrographs of HUVEC tubule formation after 4 hours on Matrigel after 24 hours pretreatment with CM from control and 40nM DHT-treated fibroblasts from young and old men. Bar, 50 μm. C, Relative absorbance of HUVECs incubated in young and old DHT-treated fibroblast CM after 24 hours; n = 3 donors for young and old performed in quadruplicate over 3 individual experiments with data presented as means ± SEM.