Table 5.

Primers Sets for Site-Directed Mutagenesis of Ccdc80-Luciferase Construct

Mutation	Forward Primer (5′–3′)	Reverse Primer (5′–3′)
Tcf site 1	GGCTCTCATACTGCGAGAAGCCGCTACTTTACCAGCTGAGG	CCTCAGCTGGTAAAGTAGCGGCTTCTCGCAGTATGAGAGCC
Tcf site 2	CCATGGGCTTTTCCAACCCAGAACTCCGCTCATAAAGCAGGCAG	CTGCCTGCTTTATGAGCGGAGTTCTGGGTTGGAAAAGCCCATGG
Tcf site 3	CGCTCGGAAACCGCTGGACTCGGCCAGACTGCAGG	CCTGCAGTCTGGCCGAGTCCAGCGGTTTCCGAGCG
Tcf site 4	CCTCATGCCTGCCGCCTGTAAATCCGCTCTGGATTTCCTGC	GCAGGAAATCCAGAGCGGATTTACAGGCGGCAGGCATGAGG

The underlined letters represent the mutations introduced to abrogate transcription factor binding.