Fig. 6.

Pax2 promoter-driven reporter activity is reduced in salt-stressed Bdkrb2^−/− metanephroi. A, C: E12.5 and E14.5 metanephroi were harvested from genetic crosses of Bdkrb2^−/−;Pax2-GFP mice subjected to gestational high salt. Photographs of GFP fluorescence (a surrogate of Pax2 promoter activity) were captured at the time of harvest. GFP fluorescence is visibly lower in Bdkrb2^−/− than Bdkrb2^+/+ metanephroi. B, D: GFP mRNA was measured by real-time quantitative RT-PCR in E12.5 and E14.5 Bdkrb2^−/− and Bdkrb2^+/+ metanephroi (n = 4–5/group).