Skip to main content
. 2014 Jun 27;3(7):644–655. doi: 10.1242/bio.20148334

Fig. 1. UE-12 replenishes the repressed expression of the lys-7 defense gene.

Fig. 1.

Representative fluorescence micrographs of the population of transgenic plys-7::gfp worms (A) fed on E. coli OP50 (uninfected), (B) infected with S. aureus in the absence of UE-12 (animals were exposed to DMSO only) and (C) infected with S. aureus in the presence of UE-12 (100× magnification). (D,E,F) The criteria used for scoring of plys-7::gfp transgene expression upon S. aureus infection in the presence and absence of extract. Each worm was given a score of 1, 2 or 3 depending on the GFP intensity observed. Asterisks mark the head of the worms. (G) Bars represent the mean ± SD of GFP intensity scores (expressed in arbitrary units) corresponding to the lys-7 expression for each worm (n = 20) according to the criteria presented in panels D, E and F. (H) qRT-PCR analysis of lys-7 mRNA shows significant induction of lys-7 expression in infected animals following treatment with UE-12 at 24 and 48 hpi. **A significant difference between untreated (DMSO) and UE-12-treated worms (p<0.001).