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Schematic depiction of the two most abundant modified nucleotides in spliceosomal snRNA. (Top) Pseudouridine is a rotational isomer of uridine, in which the N-C glycosidic bond is broken to form an C-C bond. This results in the presence of an extra hydrogen bond donor (d), while the number of hydrogen bond acceptors (a) is unchanged. (Bottom) Schematic representation of a 2'-O-methylated ribose.
