Figure 1.

Changes of transgene frequency as a function of generation number in population cage experiments. An equal number (50 virgin males and 50 virgin females) of transgenic and nontransgenic mosquitoes were placed in a cage to initiate the experiment. At each generation, transgene frequency was assessed by measuring the presence or absence of the transgene marker protein (GFP) with a fluorescence microscope. The experiment had 2 replicates.