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. Author manuscript; available in PMC: 2014 Sep 4.
Published in final edited form as: Cell Stem Cell. 2009 Jul 2;5(1):54–63. doi: 10.1016/j.stem.2009.05.003

Figure 1. Assays for Fate of hMSCs Infused into Mice.

Figure 1

(A) Clearance of human Alu sequences from blood after i.v. infusion of about 2 × 106 hMSCs into mice. Values are means ± SD; n = 6.

(B) Standard curves for real-time PCR assays of human Alu sequences in seven organs. Values indicate ΔΔCt for primers for mouse/human GAPDH genes and Alu sequences on same samples.

(C) Tissue distribution of human Alu sequences 15 min after i.v. infusion of about 2 × 106 hMSCs into mice. Values are means ± SD; n = 6.

(D) Standard curves for real-time RT-PCR assays of human mRNA for GAPDH. Values indicate ΔΔCt for primers for mouse/human GAPDH genes and cDNA for human-specific GAPDH on the same samples.

(E) Kinetics of hMSCs in lung and six other tissues after i.v. infusion of about 2 × 106 hMSCs. Values are means ± SD; n = 6.

(F) Appearance of hMSCs in heart after i.v. infusion of about 1 × 106 hMSCs 1 day after permanent ligation of the left anterior descending coronary artery.