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. 2014 Aug 31;9:2. doi: 10.1186/1747-1028-9-2

Figure 2.

Figure 2

Knockdown effect of Smurf2 siRNA in MDA-MB-231 and MCF-7 cells. (A) MDA-MB-231 cells were transfected with Smurf2 siRNA (siSmurf2) and control siRNA (siControl) at a concentration of 80 pmols. Cells were harvested 36 hours after the transfection, and the silencing effect at the Smurf2 mRNA level was determined using real-time RT-PCR. (B) MCF-7 cells were transfected with Smurf2 siRNA, and control siRNA. The silencing effect at the Smurf2 mRNA level was measured using real-time RT-PCR. The silencing effect of Smurf2 siRNA at the protein level was determined 48 hours post-transfection using western blot in (C) MDA-MB-231 cells and (D) MCF-7 cells.