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. 2014 Sep 4;5:424. doi: 10.3389/fpls.2014.00424

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Copyright © 2014 Powikrowska, Oetke, Jensen and Krupinska.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Visualization of plastid nucleoids by using different microscopic techniques. (A) Nucleoids visualized by fluorescence microscopy of SYBR Green in leaf sections, bar: 10 μm. (B) Conventional electron micrographs showing nucleoids with DNA filaments in mesophyll chloroplasts. (C) Specimen prepared by high pressure freezing and freeze substitution (HPF-FS). (D) Immunogold labeling of nucleoids in leaf sections obtained from specimen prepared by high pressure freezing and freeze substitution (HPF-FS) using a DNA specific antibody, bar: 500 nm.