Figure 5.

Localization of various CTS mutant versions of the mitochondrial-TA protein TraB in BY-2 cells. Shown on the left are schematic illustrations of wild-type and various CTS mutant versions of TraB and their corresponding localization (or lack thereof) to mitochondria in transformed BY-2 cells. The names of the mutant constructs represent the specific amino acids in their modified CTSs. All constructs possess an N-terminal-appended Myc-epitope tag. Shown for each construct is the corresponding C-terminal amino acid sequence, including putative TMD (underlined) and modified (or wild-type) CTS; additional amino acid residues inserted into the TraB CTS (i.e., threonines) are bolded. Mitochondrial localization (indicated as “Yes” or “No”) was assessed based on colocalization (or lack thereof) of the expressed protein and endogenous mitochondrial CoxII. Shown on the right in both are representative immuno-epifluorescence micrographs illustrating the localization of the various constructs shown on the left. Each micrograph is labeled with the name of the expressed Myc-tagged wild-type TraB or CTS mutant version of TraB, or endogenous CoxII. Bar = 10 μm.