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. 2014 Sep 4;5:426. doi: 10.3389/fpls.2014.00426

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Copyright © 2014 Marty, Teresinski, Hwang, Clendening, Gidda, Sliwinska, Zhang, Miernyk, Brito, Andrews, Dyer and Mullen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Localization of various CTS mutant versions of the mitochondrial-TA hybrid protein TraBΔCb5-6^CTS in BY-2 cells. Shown on the left are schematic illustrations of wild-type and various CTS mutant versions of the hybrid protein TraBΔCb5-6^CTS and their corresponding localization (or lack thereof) to mitochondria in transformed BY-2 cells. The names of the mutant constructs represent the specific amino acids in their CTSs. All constructs possess an N-terminal-appended Myc-epitope tag. Shown for each construct is the corresponding C-terminal amino acid sequence, including putative TraB TMD (underlined) and modified (or wild-type) Cb5-6 CTS; modified or additional amino acid residues inserted into the Cb5-6 CTS are bolded. Mitochondrial localization (indicated as “Yes” or “No”) was assessed based on colocalization (or lack thereof) of the expressed protein and endogenous mitochondrial CoxII. Shown on the right in both are representative immuno-epifluorescence micrographs illustrating the localization of the various constructs shown on the left. Each micrograph is labeled with the name of the expressed Myc-tagged TraBΔCb5-6^CTS or CTS mutant version of TraBΔCb5-6^CTS, or endogenous CoxII. Bar = 10 μm.