Figure 7.

Localization of various CTS mutant versions of mitochondrial-TA proteins in BY-2 cells. Shown on the left are schematic illustrations of wild-type and/or various CTS mutant versions of TraB, Cb5-6, or PMD2 and their corresponding localization (or lack thereof) to mitochondria in transformed BY-2 cells. The names of the mutants represent the specific amino acids in their modified CTSs. All constructs also possess an N-terminal-appended Myc-epitope tag. Shown for each construct is the corresponding C-terminal amino acid sequence, including putative TMD (underlined) and modified (or wild-type) CTS from TraB, Cb5-6 and PMD2; modified amino acid residues in the protein's CTS are bolded. Mitochondrial localization (indicated as “Yes” or “No”) was assessed based on colocalization (or lack thereof) of the expressed protein and endogenous mitochondrial CoxII. Shown on the right in both are representative immuno-epifluorescence micrographs illustrating the localization of the various constructs shown on the left. Each micrograph is labeled with the name of the expressed Myc-tagged wild-type and/or CTS mutant version of TraB, Cb5-6, or PMD2 or endogenous CoxII. Bar = 10 μm.