Figure 2.

Lipid binding to membrane protein complexes studied by MS. A sulfolipid was shown to bind specifically in the inner membrane ring of cATPase. The upper left model shows how the lipid is attached to the membrane ring subunit. Cardiolipins were found to bind to the membrane ring of V‐type ATPase in E. hirae. The ‘lipid plug’ reduces the inner cavity of the membrane ring to stabilise binding of the central stalk (upper right panel). MS reveals that the ABC transporter P‐gp binds up to six negatively charged diacylglycerides (e.g. POPA; lower right panel), which bind more favourably than zwitterionic lipids (e.g. DSPC; lower left panel).