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. 2014 Sep 4;8:89. doi: 10.3389/fnana.2014.00089

FIGURE 2.

FIGURE 2

Cre-recombinase in inhibitory and pyramidal neurons. (A) In situ hybridization image showing tdTomato expressing neurons in red, Gad67 positive neurons in green, and in yellow neurons expressing both mRNAs (highlighted by white arrows; image from Allen Mouse Brain Connectivity Atlas, experiment 180304494, image 33). Scale bar is 100 μm. (B) Expression of tdTomato in other inhibitory neurons. First row: immunostained sagittal sections from V1 showing no colocalization between tdTom and parvalbumin (PV). Second row: immunostained sagittal sections from V1 showing low colocalization of tdTom and somatostatin (SST) protein (white arrow). Scale bar is 100 μm. (C) Expression of tdTomato in pyramidal neurons. Immunostained coronal slices from V1 for the pyramidal marker SatB2. Red arrows show cells that are positive only for tdTomato, yellow arrows show cells that express both proteins. Scale bar is 100 μm. (D) Close up of a tdTomato neuron with a pyramidal shape. Scale bar is 100 μm.