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. 2014 Aug 19;5:406. doi: 10.3389/fpls.2014.00406

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Copyright © 2014 Creason, Davis, Putnam, Vandeputte and Chang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cloud plot of ANIb vs. TETRA for all possible pairwise comparisons. Average nucleotide identity (ANIb; x-axis) and tetranucleotide (TETRA; y-axis) usage patterns were calculated and plotted as a factor of isolate grouping (z-axis). All pairwise comparisons, including reciprocal comparisons are presented, with colors assigned based on the clade membership of the isolate being compared to. Gray colored areas demark 96% ANI and 0.997 TETRA thresholds. Clouds are demarcated by dotted lines. Circles with black borders are below the TETRA threshold. Circles with yellow borders (comparisons between isolates of sub-clade iii; purple) exceed the TETRA threshold but not the ANIb threshold. The black circle represents a comparison between R. jostii RHA1 and R. equi 103S.