Table 2.
Human Aβ and mouse endogenous tau ELISA results in the 5XFAD Tg mice.
| PROTEIN | MEAN CONCENTRATION | SEM | p = |
|---|---|---|---|
| Tris-soluble Aβ40 (pg/mg total protein) | 0.0296 | ||
| 3 months | 101 | 11 | |
| 6 months | 271 | 75 | |
| 9 months | 386 | 66 | |
| Tris-soluble Aβ42 (pg/mg total protein) | 0.0091 | ||
| 3 months | 25 | 25 | |
| 6 months | 354 | 77 | |
| 9 months | 627 | 84 | |
| GHCl-soluble Aβ40 (ng/mg total protein) | 0.0183 | ||
| 3 months | 13 | 4 | |
| 6 months | 87 | 27 | |
| 9 months | 132 | 20 | |
| GHCl-soluble Aβ42 (ng/mg total protein) | 0.0154 | ||
| 3 months | 199 | 42 | |
| 6 months | 1133 | 281 | |
| 9 months | 1971 | 376 | |
| Total Aβ (ng/mg total protein) | 0.0125 | ||
| 3 months | 338 | 80 | |
| 6 months | 1844 | 459 | |
| 9 months | 3114 | 500 | |
| Tris-soluble tau (ng/mg total protein) | 0.2866 | ||
| 3 months | 52 | 10 | |
| 6 months | 76 | 10 | |
| 9 months | 70 | 11 | |
| GHCl-soluble tau (ng/mg total protein) | 0.6939 | ||
| 3 months | 200 | 21 | |
| 6 months | 180 | 16 | |
| 9 months | 197 | 11 | |
| Total tau (ng/mg total protein) | 0.7788 | ||
| 3 months | 252 | 31 | |
| 6 months | 255 | 12 | |
| 9 months | 267 | 21 |
Formulations: Tris = 20 mM Tris-HCl, 5 mM EDTA, pH 7.8 buffer; GHCl = 5 M guanidine hydrochloride, 50 mM Tris-HCl, pH 8.0 buffer. Total tau is the sum of Tris- and GHCl-soluble tau. Statistical analyses were performed using a Kruskal–Wallis test. Total Aβ is the sum of Aβ40 and Aβ42 for both Tris- and GHCl-soluble fractions.
Abbreviations: Aβ, amyloid-beta; SEM, standard error of the mean.