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. Author manuscript; available in PMC: 2014 Sep 4.
Published in final edited form as: J Cell Biochem. 2013 Jun;114(6):1217–1222. doi: 10.1002/jcb.24471

Figure 1. Estrogen inhibits miR-21 biogenesis.

Figure 1

(A) RT-PCR analysis of ERα expression in RANKL-induced osteoclastogenesis for the indicated days. (B) QRT-PCR analysis of FasL expression in RANKL-induced osteoclastogenesis for the indicated days. (C) Immunoblotting analysis of FasL expression during RANKL-induced osteoclastogenesis with E2 treatment for the indicated days. (D-F) MiR QRT-PCR analysis of pre-miR-21/miR-21 expressions in RANKL-induced osteoclastogenesis with E2 treatment for the indicated days. (D) and (E): ERα-wt cells, (F): ERα-null cells. (G and H) ChIP assay was performed to study the association of c-Fos with miR-21 promoter during RANKL-induced osteoclastogenesis with E2 treatment for the indicated days. (G): ERα-wt cells, (H): ERα-null cells.