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. Author manuscript; available in PMC: 2014 Sep 4.
Published in final edited form as: J Cell Biochem. 2013 Jun;114(6):1217–1222. doi: 10.1002/jcb.24471

Figure 2. Mir-21 overexpression rescues osteoclastic apoptosis induced by estrogen.

Figure 2

(A) MiR QRTPCR analysis of miR-21 expression in M-CSF-dependent BMMs. (B) TRAP-positive osteoclasts counted at 3 days after RANKL treatment, cultured in 24-well plates. Similar findings were obtained in 4 independent sets of experiments. (C and D) Immunoblotting analysis of FasL (C) and caspase-3/cleaved caspase-3 expression (D) in RANKL-induced osteoclastogenesis (C) and in osteoclasts (D) with E2 treatment. (E) TRAP-positive osteoclasts counted at 3 days after RANKL treatment, cultured in 24-well plates. Similar findings were obtained in 4 independent sets of experiments. (F) Immunoblotting analysis of FasL expression in osteoclasts with E2 treatment. (G) Caspase-3 activity assay in osteoclasts with E2 treatment.