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. Author manuscript; available in PMC: 2014 Sep 4.
Published in final edited form as: Nat Genet. 2012 Sep 23;44(11):1243–1248. doi: 10.1038/ng.2414

Figure 3.

Figure 3

Protein blot of lymphoblastoid cell lines (LCLs). Protein blot analysis of ADAR1 expression in Epstein-Barr virus (EBV)-transformed LCLs from one unrelated control and two affected individuals (AGS93, AGS107). Whole-cell lysates were derived from 1 × 107 cells per sample, and 10 μg of total protein was loaded per lane. To test antibody specificity and confirm IFN induction of the p150 isoform of ADAR1, unstimulated cells were compared to IFN-stimulated cells. The antibody to ADAR1 recognizes both the p110 and p150 isoforms. Immunoblotting of tubulin (50–55 kDa) was used as a loading control.