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. 2014 Aug 27;13:113. doi: 10.1186/s12934-014-0113-4

Table 2.

Nucleotide sequences of primers used for PCR and construction of Ub::hGH fusion gene

Name of primer DNA sequence (restriction sites underlined) T m (°C) Restriction site
hGHubF GGGGCCGCGGTTCCCAACCATTCCCTTAAGTAGGC 57 R.SacII
hGHubR GGGGGTCGACTTAGAAGCCACAGCTGCCCTCC 58 R.SalI