Figure 1.

Representative phase contrast (upper panel) and fluorescent microscopy (lower panel) images of recombinant L. salivarius cells. (A) Control cells pNZ:8048. (B) L. salivarius GFP cells displayed fluorescence observed throughout individual bacilli. (C) Negligible levels of fusion fluorescence were observed from MMP-GFP cells, (D) while fluorescent foci, suggestive of aggregation, were observed toward the polar regions of individual cells for the codon optimized variant MMPsynth-GFP. (E) Low levels of fluorescence prevented determination of the subcellular localization of native MptD-GFP fusions within L. salivarius cells, while (F) codon optimized MptDsynth-GFP fusion proteins demonstrated the tendency to localize toward the cellular membrane periphery. All culture assays were performed in triplicate, multiple images were taken from each sample and representative pictures were chosen. Bars represent 5 μm.