Table 1.
Levels of monoamines and their metabolites detected ex vivo in cortex, bulbs, hypothalamus, striatum, hippocampus, and cerebellum (mean ± SEM: picogram per milligram of wet tissue).
| Brain region | Genotype | Neurotransmitter, metabolite concentration picogram per milligram wet brain weight) |
||||||
|---|---|---|---|---|---|---|---|---|
| Noradrenergic system |
Dopaminergic system |
Serotoninergic system |
||||||
| NA | MOPEG | DA | DOPAC | HVA | 5-HT | 5-HIAA | ||
| Cortex | Wt | 469.60 ± 22.99 | 102.74 ± 7.53 | 874.14 ± 268.95 | 134.50 ± 19.85 | 252.97 ± 35.58 | 523.10 ± 29.72 | 341.42 ± 17.59 |
| Het | 440.84 ± 28.76 | 102.47 ± 6.20 | 296.37 ± 69.32* | 108.65 ± 18.65 | 184.52 ± 40.76 | 470.09 ± 31.15 | 285.37 ± 14.32 | |
| Bulbs | Wt | 291.82 ± 24.28 | 46.35 ± 5.94 | 312.11 ± 33.66 | 100.26 ± 8.63 | 152.87 ± 15.49 | 247.65 ± 25.63 | 191.88 ± 16.88 |
| Het | 346.10 ± 14.98 | 66.26 ± 6.28 | 396.15 ± 23.84* | 102.06 ± 6.83 | 154.78 ± 8.95 | 131.53 ± 20.97 | 192.13 ± 9.76 | |
| Hypothalamus | Wt | 1789.74 ± 80.36 | 205.08 ± 23.06 | 394.87 ± 16.83 | 116.63 ± 6.12 | 362.10 ± 13.19 | 518.33 ± 22.43 | 934.82 ± 42.78 |
| Het | 1742.52 ± 81.41 | 183.74 ± 7.50 | 540.86 ± 163.65 | 144.44 ± 5.48** | 444.55 ± 13.27** | 487.36 ± 29.79 | 864.22 ± 19.73 | |
| Striatum | Wt | 129.71 ± 32.78 | 339.05 ± 49.89 | 12521.13 ± 1661.54 | 2718.95 ± 326.72 | 4310.08 ± 383.59 | 453.07 ± 30.88 | 654.30 ± 23.09 |
| Het | 128.12 ± 19.16 | 261.70 ± 25.90 | 12275.97 ± 1376.40 | 2847.63 ± 196.75 | 4583.34 ± 398.19 | 109.34 ± 26.48 | 679.84 ± 24.65 | |
| Hippocampus | Wt | 508.02 ± 33.64 | 115.37 ± 6.77 | 82.16 ± 7.61 | 18.21 ± 1.33 | 69.47 ± 8.48 | 505.52 ± 61.78 | 540.99 ± 37.90 |
| Het | 423.67 ± 39.12 | 108.17 ± 8.75 | 84.80 ± 13.48 | 25.16 ± 4.81 | 42.01 ± 6.93* | 580.54 ± 33.18 | 503.31 ± 53.05 | |
| Cerebellum | Wt | 402.46 ± 28.48 | 48.85 ± 3.12 | 5.09 ± 1.06 | 12.52 ± 2.38 | 80.66 ± 5.09 | 115.60 ± 17.51 | 129.05 ± 6.03 |
| Het | 433.56 ± 17.08 | 46.17 ± 3.42 | 6.75 ± 2.68 | 18.77 ± 7.55 | 79.52 ± 3.59 | 118.94 ± 21.13 | 126.85 ± 6.04 | |
| Brain region | Genotype |
Neurotransmitter turnover |
||||||
| NA | DA | 5-HT | ||||||
| Cortex | Wt | 0.22 ± 0.01 | 0.24 ± 0.05 | 0.66 ± 0.03 | ||||
| Het | 0.24 ± 0.01 | 0.45 ± 0.05* | 0.62 ± 0.03 | |||||
| Bulbs | Wt | 0.16 ± 0.02 | 0.33 ± 0.02 | 0.80 ± 0.05 | ||||
| Het | 0.19 ± 0.01 | 0.26 ± 0.02* | 0.86 ± 0.05 | |||||
| Hypothalamus | Wt | 0.11 ± 0.01 | 0.30 ± 0.02 | 1.81 ± 0.09 | ||||
| Het | 0.11 ± 0.01 | 0.35 ± 0.04 | 1.83 ± 0.11 | |||||
| Striatum | Wt | 4.09 ± 1.01 | 0.23 ± 0.03 | 1.47 ± 0.06 | ||||
| Het | 2.86 ± 0.7E | 0.26 ± 0.03 | 1.71 ± 0.10 | |||||
| Hippocampus | Wt | 0.23 ± 0.01 | 0.23 ± 0.02 | 0.95 ± 0.11 | ||||
| Het | 0.26 ± 0.02 | 0.29 ± 0.02* | 0.87 ± 0.07 | |||||
| Cerebellum | Wt | 0.12 ± 0.01 | 2.83 ± 0.51 | 1.25 ± 0.13 | ||||
| Het | 0.11 ± 0.01 | 6.76 ± 2.45 | 1.36 ± 0.23 | |||||
*P < 0.05, **P < 0.005 between wildtype and heterozygous reeler mice.