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. Author manuscript; available in PMC: 2014 Dec 13.
Published in final edited form as: Nat Commun. 2014 Jun 13;5:4137. doi: 10.1038/ncomms5137

Figure 1. Abundance of Pol II complexes at Igh-V region in Ramos cells.

Figure 1

a) The structure of the Igh-V region in the reporter Ramos cell line is depicted with grey bars indicating the positions of each primer set used in the following figures and rectangular boxes representing coding exons; b) Chromatin immunoprecipitation (ChIP) was conducted on reporter cells expressing the AID-ER fusion protein but without 4-OHT treatment using anti-total Pol II, quantified by real-time PCR and normalized to the level at Cμ region after subtraction of IgG background. Average total-Pol II occupancy at Igh-V region and downstream regions were compared using student T test; c) The abundance of Pol II at each region shown in a; d) ChIP was conducted on reporter cells using anti-Pol II S5P was quantified by real-time PCR. Data were then normalized to signals at the Cμ region after subtraction of IgG background. Data represent the average of two independent experiments and error bars represent SD.