Table I.
Proteins Associated with Lipoplex Formulations. Proteins associated with each formulation were identified by MALDI/MS. The 50:50 formulation adsorbed many additional proteins, but only those in common with the other formulations are listed for comparison. The total quantity of adsorbed protein on each formulation is also shown, as is the diameter (nm) of each lipoplex before and after incubation in fetal calf serum (FCS).
| 24PC | 22PC | 20PC | 18PC | 16PC | 14PC | 20:80 | 50:50 | |
|---|---|---|---|---|---|---|---|---|
| Unidentified (3,530 Da) | - | - | - | x | x | x | x | x |
| Unidentified (12,600 Da) | - | - | - | x | x | x | - | - |
| Unidentified (13,300 Da) | x | x | x | - | - | - | - | x |
| Cationic trypsin (25,768 Da) | - | - | - | - | - | - | x | x |
| Immunoglobin gamma-2 C region (35,878 Da) | x | x | x | - | - | - | x | x |
| Immunoglobin gamma-1 C region (36,083 Da) | x | x | x | - | - | - | x | x |
| Alpha-2-HS-glycoprotein (38,394 Da) | x | x | x | - | - | - | - | x |
| Albumin (69,248 Da) | - | - | - | x | x | x | x | x |
| Collagen alpha-1 chain (93,594 Da) | x | x | x | - | - | - | - | - |
| mg protein/g lipid | 185.5 | 107.6 | 155.1 | 131 | 250.3 | 229.6 | 76.4 | 181.1 |
| diameter before FCS | 174.5 | 178.4 | 162.4 | 204.7 | 293.4 | 290.5 | 209.1 | 204.6 |
| diameter after FCS | 189.7 | 182.6 | 158.5 | 535.4 | 419 | 546.5 | 217.3 | 219.6 |