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. 2014 Sep 1;64(3):213–221. doi: 10.1270/jsbbs.64.213

Fig. 4.

Fig. 4

Expression patterns of WFT in non-vernalized WT and exe mutants plants at the 1-leaf stage to 6-leaf stages. The data at the 2-leaf stage are missing. The level of WFT expression was determined by real-time PCR. The Ubiquitin (Ubi-1) gene was used as an internal control for calculating the relative levels of expression of the WFT gene. Means and standard errors are indicated. (A) Expression patterns in seedlings at each leaf stage under LD conditions. Leaf stage numbers (1L, 3L, etc.) are shown for exe4 only. (B) Expression patterns in seedlings at each leaf stage under SD conditions. Leaf stage numbers (1L, 3L, etc.) are shown for exe4 only. ANOVA (analysis of variance) was performed to detect the difference of gene expression level at the 3L and 4L stages. The same superscript means not significantly different (p = 0.05) from each other when Fisher’s LSD (least significant difference) analysis is applied.