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Flow cytometry measurement of uptake of PK NPs and LPK NPs by JAWSII DCs. One milligram of NPs was incubated with 106 cells for 1, 2, and 3 h, respectively. As time lapsed, more NPs were ingested by cells. Enhanced uptake of LPK NPs by DCs was observed compared to PK NPs. DCs are more readily to uptake positively charged NPs compared to negatively charged NPs. Most of the cells (>90%) had taken up LPK NPs in 3 h, while only 52% of the cells had taken up PK NPs.
