Figure 7. ¹³C MFA Flux Map for D. vulgaris and M. maripaludis co-culture grown on lactate using peptide-based method for 10 amino acids and 20 peptides.

The peptide-based method was able to recover the initial FBA flux profile used to derive peptide labeling, showing the feasibility of this method for a simple community. Lactate uptake flux was set as 1 µM/h; acetate, formate and CO₂ were produced at a rate of 0.87 µM/h, 0.28 µM/h and 0.63 µM/h respectively. Best fit for flux is given on top red number for each reaction. Reversible reactions are indicated by double arrows.