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. 2014 Sep 4;8(9):e3119. doi: 10.1371/journal.pntd.0003119

Figure 3. Mechanism of CFTR inhibition by diclofenac in T84 cells.

Figure 3

(A) Schematic diagram illustrating the regulatory mechanisms of CFTR Cl− channel activity in T84 cells. Inhibitors/activators used in this study are shown. PP, protein phosphatase; AMPK, AMP-activated protein kinase; PDE, phosphodiesterase; PKA, protein kinase A; AC, adenylate cyclase; MRP4, multidrug resistance-associated protein 4 (B) Involvements of PDE, MRP4, AMPK and protein phosphatase in the inhibition of CFTR-mediated Cl− transport. Dose-inhibition studies of diclofenac were performed after the indicated treatments. Representative current tracings are shown. (C) Summary of dose-inhibition studies. Data are fitted to Hill equation and expressed as means of % agonist-stimulated apical Cl− current ± S.E.M. (n = 6–8).