Table 1. Listing of predicted B. malayi NMT substrates.

B. malayi locus	Myristoylation motif	Score	Protein information	C. elegans homolog
Bm1_33335	GGNNSTPKSDTMMDHQP	3.982	hypothetical protein	F46C8.3
Bm1_01010	GCTMSQEERAALERSRM	3.961	guanine nucleotide-binding protein G-o protein alpha subunit	C26C6.2 *
Bm1_20595	GNYKSRPPSSCADELKK	3.617	Protein kinase domain containing protein	C24A1.3a *
Bm1_07670	GGRQSSQVSHEVAQSEV	3.327	cytochrome C-type heme lyase	T06D8.6 *
Bm1_36040	GNCFSGSPRQSSPSSDI	2.626	SRC-1, putative	Y92H12A.1 *
Bm1_54125	GNSGSTNIPGGGTEGYH	2.584	golgi reassembly stacking protein 2, putative	Y42H9AR.1
Bm1_51340	GSCQSQEAQEQLARNKA	2.509	Guanine nucleotide-binding protein alpha-3 subunit, putative	C34D1.3 *
Bm1_52520	GQQQSGFGGKREDRGGG	2.411	Probable 26S protease regulatory subunit 4	F29G9.5 *
Bm1_44925	GNALCCLQDSSSAARSK	2.368	cyclin fold protein 1 variant b	ZK353.1b
Bm1_35125	GCVNSIDQNAKARSKQI	2.304	GTP-binding regulatory protein alpha chain - starfish, putative	T07A9.7
Bm1_03585	GQIASIRRRASVPNVTN	2.205	hypothetical protein	C11H1.3 *
Bm1_15680	GSSQSYEESNRSDDLPK	1.780	hypothetical protein	M176.3 *
Bm1_01525	GQERSILSRKSQNTIEM	1.736	hypothetical protein	F54B11.5
Bm1_53985	GASLTSPLPHRPPTSVC	1.705	hypothetical protein Bm1_53985	T23F11.3b
Bm1_32000	GNGQSSNDTNIPSSHSF	1.704	hypothetical protein	F59E12.11 *
Bm1_45820	GGRCSRRAPSPTHPKLS	1.688	Protein kinase domain containing protein	ZK909.2h *
Bm1_26080	GQFLSTTASVDEESILR	1.646	Glutaredoxin family protein	ZK121.1a *
Bm1_16785	GNTGSSLTDLNLFSKGG	1.635	calcium and integrin binding family member 2	F30A10.1 *
Bm1_28345	GAYLSKPITEKISECGG	1.596	Protein phosphatase 2C containing protein	T23F11.1
Bm1_34880	GAATSGLQSQMQQEHDP	1.384	LD28933p	F20D1.1
Bm1_31655	GQQQSAFVVEIASSRFG	1.301	hypothetical protein	Y67A10A.7
Bm1_07365	GKAGSKVKNYSKIKKNN	1.146	TLD family protein	K08E7.1 *
Bm1_41730	GSCLGKKSSTTILEAVH	0.948	protein-tyrosine kinase	F49B2.5 *
Bm1_38795	GLTISGLFGRLFGKKQV	0.755	ADP-ribosylation factor 4	F57H12.1 *
Bm1_55795	GTRLSVSLEDPEVSPRT	0.189	Probable NADH-ubiquinone oxidoreductase B18 subunit	D2030.4 *
Bm1_04790	GISTSMQKDSTVLSCYG	0.183	Platelet-activating factor acetylhydrolase	C52B9.7a *
Bm1_46940	GNAAGSIKRSKSIGTWI	0.105	hypothetical protein	C52A11.2 *
Bm1_12460	GNLFGKQRPALTPVSQQ	0.076	FLJ11749-like	Y65B4A.3 *
Bm1_28970	GTTIAIKRKGTGESGKS	−0.192	G-protein alpha subunit, putative	C26C6.2 *
Bm1_51315	GICQSQEEKTMVAKSRA	−0.743	Guanine nucleotide-binding protein alpha-3 subunit, putative	E02C12.5 *
Bm1_36120	GIKSSKPKLSKEDLDFL	−0.798	Neuronal calcium sensor 2	F10G8.5 *
Bm1_17230	GNRESSTSLTSLTSLIS	−0.812	Ubiquitin carboxyl-terminal hydrolase family protein	ZK328.1b *
Bm1_54455	GKLLSKIFGKREMRILM	−1.097	ADP-ribosylation factor 6	B0336.2 *
Bm1_39660	GNMEASGREMDNPDAEE	−1.101	map kinase activated protein kinase protein 2, isoform b	F42G8.3b *
Bm1_43750	GAKISSETLIEENTYLR	−1.488	RIKEN cDNA 4933427L07	C47D12.2 *
Bm1_49765	GADGGTIPKRCELVKKK	−1.607	chromosome 20 open reading frame 43	C01A2.5 *
Bm1_50685	GALLATPACISSLACCC	−1.798	TDE2 protein	Y57E12AL.1a*
Bm1_18185	GNNQGGLHKRERALDGQ	−1.911	5′-AMP-activated protein kinase, beta subunit	Y47D3A.15

Survey of potential targets for N-terminal glycine myristoylation in B. malayi. Predicted myristoylated proteins in the proteome of C. elegans (145 clusters) were retrieved from MYRbase and used to query the B. malayi genome. Homologs with a BLASTP E-Value <e⁻¹⁰ were analyzed using the MYR predictor [55] to predict myristoylation sites. Protein sequences that were scored as “Reliable” with a positive score or “Twilight zone” with a negative score were retained and duplicates were discarded. C. elegans homologs that showed any abnormal phenotype in WormBase were marked with *. Unlikely candidates such as multi-pass integral membrane proteins were removed.