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. 2014 Sep 4;9(9):e106661. doi: 10.1371/journal.pone.0106661

Figure 5. Whsc1 is required for Runx2 binding, H3K36me3, and H4 acetylation of the Opn and Col1a1 genes.

Figure 5

(A–F) Runx2, H3K36me3, and H4ac occupancy was examined by ChIP qPCR in MC3T3-E1 cells differentiated by 6 days incubation with BMP-2. (A) Runx2, H3K36me3, and H4ac occupancy in wild type MC3T3-E1 cells. Runx2 (B), H3K36me3 (C, D) and H4Ac (E, F) occupancy at the indicated regions of the Opn and Col1a1 genes were examined in MC3T3-E1 cells transfected with the indicated siRNAs. The results are relative to 2% input and normalized to H1foo. Error bars indicate the s.d. (n = 3). *, P<0.05.