Figure 1. Multiple alignment of the deduced amino acid sequence of mature NfBGL1 (XP_001261562) with other fungal counterparts from T. reesei (TrBgl1, 1713235A), and A. aculeatus (AaBGL1, 4IIB).

Identical and similar amino acids are indicated by solid black and gray boxes, respectively. The putative catalytic residues, D235 and E447, are indicated with asterisks. The residues probably related to subsite −1 (W236) and +1 (W36, N261, and Y449) are indicated with white and black triangles, respectively. The residues probably forming hydrogen bonds with substrate, D60, R124, K147 and H148, are indicated with black dots.